Fabrication of Electrospun Membranes based on Poly(caprolactone) (PCL) and PCL/Chitosan Layer by Layer for Tissue Engineering

Authors

  • Choi Yee Foong Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Naznin Sultana Advanced Membrane Technology Research Centre (AMTEC), Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/amst.v17i1.12

Abstract

Recently, in the field of tissue engineering, fabrication of three-dimensional (3D) electrospun scaffold or membrane is much emphasized. In this study, layered composite scaffolds or membranes were fabricated using two biodegradable polymers, polycaprolactone (PCL) and Chitosan layer-by-layer with multilayer electrospinning method. Characterizations of membranes were done using several techniques. Electrospun composite membrane’s surface morphology was examined using a Scanning Electron Microscopy (SEM) and the wettability of the material’s surface was determined using water contact angle measuring measurement (WCA). Water uptake properties of electrospun membrane were also determined. Using optimized solution concentration and electrospinning processing parameters, the composite PCL/Chitosan and PCL layer-by-layer were successfully fabricated. It was observed from SEM that the composite electrospun membranes produced consisted microfibers and nanofibers within single scaffold. The water contact angle for the double-layered composite electrospun membranes was lower than the pure PCL. The double-layered composite membrane also had higher water uptake properties compared to pure PCL scaffold.

References

Chen, A. A., V. L. Tsang, D. R. Albrecht, and S. N. Bhatia. 2007. 3-D fabrication technology for tissue engineering. In BioMEMS and Biomedical Nanotechnology. Springer. 23–38.

Karp, J. M., P. D. Dalton, and M. S. Shoichet. 2003. Scaffolds for tissue engineering. MRS Bulletin. 28(04): 301–306.

Leor, J., Y. Amsalem, and S. Cohen. 2005. Cells, scaffolds, and molecules for myocardial tissue engineering. Pharmacology & therapeutics. 105(2): 151–163.

Nair, L. S. and C. T. Laurencin. 2007. Biodegradable Polymers as Biomaterials. Progress in Polymer Science. 32(8): 762–798.

Vroman, I. and L Tighzert. 2009. Biodegradable polymers. Materials. 2(2): 307–344.

Gomes, M. E., H. L. Holtorf, R. L. Ries, and A. G. Mikos. 2006. Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. Tissue Engineering. 12(4): 801–809.

Tuzlakoglu, K., N. Bolgen, A. J. Salgodam, M. E. Gomes, E. Piskin, and R. L. Reis. 2005. Nano-and micro-fiber combined scaffolds: a new architecture for bone tissue engineering. Journal of Materials Science: Materials in Medicine. 16(12): 1099–1104.

Mota, C., D. Puppi, D. Dinucci, C. Errico, P. Bartolo, and F. Chiellini. 2011. Dual-scale polymeric constructs as scaffolds for tissue engineering. Materials. 4(3): 527–542.

Puppi, D., A. M. Piras, F. Chiellini, E. Chieillini, A. Martin, I. B. Leonor, N. Neves, and R. Peis. 2011. Optimized electroâ€and wetâ€spinning techniques for the production of polymeric fibrous scaffolds loaded with bisphosphonate and hydroxyapatite. Journal of Tissue Engineering and Regenerative Medicine. 5(4): 253–263.

Middleton, J. C. and A. J. Tipton. 2000. Synthetic Biodegradable Polymers as Orthopedic Devices. Biomaterials. 21(23): 2335–2346.

Gunatillake, P. A. and Adhikari, R. 2003. Biodegradable synthetic

polymers for tissue engineering. Eur Cell Mater. 5(1): 1–16.

Sarasam, A. and Madihally, S. V. 2005. Characterization of chitosan–polycaprolactone blends for tissue engineering applications. Biomaterials. 26(27): 5500–5508.

Suyatma, N. E., A. Copinet, L. Tighzert, and V. Coma. 2004. Mechanical and barrier properties of biodegradable films made from chitosan and poly (lactic acid) blends. Journal of Polymers and the Environment. 12(1): 1–6.

Deng, D., P. Jiao, X. Ye, and L. Xia. 2012. An image-based model of the whole human heart with detailed anatomical structure and fiber orientation. Computational and Mathematical Methods in Medicine.

Butler, B., P. J. Vergano, R. F. Testin, J. M. Bunn, and J. L. Wiles. 1996. Mechanical and barrier properties of edible chitosan films as affected by composition and storage. Journal of Food Science. 61(5): 953–956.

Hosokawa, J., M. Nishiyama, K. Yoshihara, and T. Kubo. 1990. Biodegradable film derived from chitosan and homogenized cellulose. Industrial & Engineering Chemistry Research. 29(5): 800–805.

Chen, G., T. Ushida, and T. Tateishi. 2002. Scaffold design for tissue engineering. Macromolecular Bioscience. 2(2): 67–77.

Borm, P. J., D. Robbins, S. Houbold, T. Kuhlbusch, H. Fissan, K. Donaldson, R. Schins, V. Stones, W. Kreyling, and J. Lademann. 2006. The potential risks of nanomaterials. Particle and Fibre Toxicology. 3(1): 11.

Wang, Z.M. 2008. One-dimensional nanostructures. Vol. 3. Springer Science & Business Media.

Chen, H., A. G. Mikos, Q. P. Pham, U. Sharma, and Z. P. Luo. Finite Element Analyses of Flow Field in Multilayer Nanofiber/Microfiber Scaffolds.

Pham, Q. P., U. Sharma, and A. G. Mikos. 2006. Electrospun poly (ε-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules. 7(10): 2796–2805.

Kumbar, S. G., S. P. Nukavarapu, R. James, L. S. Nair, and C. T. Laurancin. 2008. Electrospun poly (lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials. 29(30): 4100–4107.

Zhang, B. 2011. Electrospun Poly (2-Hydroxyethyl Methacrylate) Nanofibrous Scaffolds for Skin Tissue Engineering. The University of Akron.

Travan, A., E. Marsich, I. Donati, M. P. Foulc, N. Moritz, H. T. Aro,

S. Paoletti. 2012. Polysaccharide-coated thermosets for orthopedic applications: from material characterization to in vivo tests. Biomacromolecules. 13(5): 1564–1572

Goddard, J. M. and J. Hotchkiss. 2007. Polymer surface modification for the attachment of bioactive compounds. Progress in Polymer Science. 32(7): 698–725.

Sultana, N. and T. H. Khan. 2013. Water absorption and diffusion characteristics of nanohydroxyapatite (nHA) and poly (hydroxybutyrate-co-hydroxyvalerate-) based composite tissue engineering scaffolds and nonporous thin films. Journal of Nanomaterials. 2013: 1–8.

Mad Jin, R., N. Sultana, S. Baba, S. Hamdan, and A.F. Ismail. 2015. Porous PCL/Chitosan and nHA/PCL/Chitosan Scaffolds for Tissue Engineering Applications: Fabrication and Evaluation. Journal of Nanomaterials. 2015: 1–8.

Downloads

Published

2017-11-13

How to Cite

Yee Foong, C., & Sultana, N. (2017). Fabrication of Electrospun Membranes based on Poly(caprolactone) (PCL) and PCL/Chitosan Layer by Layer for Tissue Engineering. Journal of Applied Membrane Science &Amp; Technology, 17(1). https://doi.org/10.11113/amst.v17i1.12

Issue

Section

Articles