Fabrication of Electrospun Membranes based on Poly(caprolactone) (PCL) and PCL/Chitosan Layer by Layer for Tissue Engineering
AbstractRecently, in the field of tissue engineering, fabrication of three-dimensional (3D) electrospun scaffold or membrane is much emphasized. In this study, layered composite scaffolds or membranes were fabricated using two biodegradable polymers, polycaprolactone (PCL) and Chitosan layer-by-layer with multilayer electrospinning method. Characterizations of membranes were done using several techniques. Electrospun composite membraneâ€™s surface morphology was examined using a Scanning Electron Microscopy (SEM) and the wettability of the materialâ€™s surface was determined using water contact angle measuring measurement (WCA). Water uptake properties of electrospun membrane were also determined. Using optimized solution concentration and electrospinning processing parameters, the composite PCL/Chitosan and PCL layer-by-layer were successfully fabricated. It was observed from SEM that the composite electrospun membranes produced consisted microfibers and nanofibers within single scaffold. The water contact angle for the double-layered composite electrospun membranes was lower than the pure PCL. The double-layered composite membrane also had higher water uptake properties compared to pure PCL scaffold.
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